Match details

Record ID:	JB089301.1
Database:	emblrelpat	Go to original record
Description:	Sequence 10 from Patent EP2503872.
Taxon:	synthetic construct
Original size:	5776 bp
Patents:	EP2503872 (lens, espacenet)

View other 16 matches for this record

Method:	QT-EVE-ZM-003	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of maize event MON87427(EURL GMFF, 2015).
Target:	MON87427

View other 2 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	926	1020	+	95 bp

Amplicon:

95 bp

1     acggaaacgg tcgggtcaaa tgtagaaaat cgggacaata tggagaaaaa
51    gaaagagtaa ttaccaatat ggagaaaacc gggaaatcta catgg

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	22 bp	0	0	1	22	4.15e-10
primerB	100.0	24 bp	0	0	95	72	3.58e-11
probe	100.0	28 bp	0	0	30	57	2.59e-13
probe	100.0	14 bp	0	0	65	78	1.57e-05

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.