Match details

Record ID:	JA070119.1
Database:	emblrelpat	Go to original record
Description:	Sequence 3 from Patent EP2298921.
Taxon:	synthetic construct
Original size:	2880 bp
Patents:	EP2298921 (lens, espacenet)

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Method:	QT-EVE-GH-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of cotton event MON88913 (Charles Delobel et al., 2009).
Target:	MON 88913

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2061	2154	+	94 bp

Amplicon:

94 bp

1     caaattaccc attaagtagc caaattacaa attttaattc aatgtagtca
51    aacactgata gtttaaacat gactctctta aggtagccaa agcc

View other 10 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	28 bp	0	0	1	28	2.56e-13
primerB	100.0	24 bp	0	0	94	71	3.54e-11
probe	100.0	22 bp	0	0	63	42	4.10e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.