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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: CS566040.1
Database: emblrelpat Go to original record
Description: Sequence 27 from Patent WO2006039376.
Taxon: synthetic construct
Original size: 2830 bp
Patents: WO2006039376 (lens, espacenet)

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Method: QL-CON-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the Cauliflower Mosaic Virus 35S promoter and the pat gene
Target:CaMV P-35S-pat

View other 205 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1872 1973 + 102 bp
Amplicon: 102 bp 
1     aagttcattt catttggaga ggacagggta cccggggatc caccatgtct
51    ccggagagga gaccagttga gattaggcca gctacagcag ctgatatggc
101   cg

View other 133 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 1.13e-11
primerB 100.0 21 bp 0 0 102 82 1.51e-09
probe 100.0 28 bp 0 0 51 78 2.64e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.