Match details

Record ID: CS566037.1
Database: emblrelpat Go to original record
Description: Sequence 24 from Patent WO2006039376.
Taxon: synthetic construct
Original size: 7390 bp
Patents: WO2006039376 (lens, espacenet)

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Method: QL-CON-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
Target:pat-CaMV T-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6824 7033 + 210 bp

Amplicon: 210 bp
1     atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51    gctcctccaa ggccagttag gccagttacc cagatctgag tcgacctgca
101   ggcatgcccg ctgaaatcac cagtctctct ctacaaatct atctctctct
151   ataataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
201   gtttcgctca  

View other 140 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.98e-09
primerB 100.0 23 bp 0 0 210 188 2.61e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.