Match details

Record ID: MO400593.1
Database: emblrelpat Go to original record
Description: Sequence 4 from patent US 10428393.
Taxon: unidentified
Original size: 200 bp
Patents: US10428393 (lens, espacenet)

Method: QT-EVE-ZM-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event 5307(EURL GMFF, 2014).
Target:Maize 5307

View other 4 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 37 143 + 107 bp

Amplicon: 107 bp
1     catggccgta tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
51    tacaccacaa tataccctct tccctgggcc aggctgggcc cactggcaaa
101   gggtgca 

View other 4 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.59e-09
primerB 100.0 18 bp 0 0 107 90 6.01e-08
probe 100.0 29 bp 0 0 54 82 8.09e-14


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.