Match details

Record ID:	MO186848.1
Database:	emblrelpat	Go to original record
Description:	Sequence 6 from patent US 10344292.
Taxon:	unidentified
Original size:	13224 bp
Patents:	US10344292 (lens, espacenet)

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Method:	QT-EVE-GM-003	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of soybean event MON87705 (Savini et al., 2012)
Target:	MON87705

View other 8 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	10680	10765	+	86 bp

Amplicon:

86 bp

1     ttcccggaca tgaagccatt tacaattgaa gagactcagg gtgttgttat
51    cactgcggtt tggcctttgg gccaaggcac cgttgt

View other 8 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	23 bp	0	0	1	23	1.10e-10
primerB	100.0	22 bp	0	0	86	65	3.73e-10
probe	100.0	30 bp	0	0	29	58	1.96e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.