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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: CQ924483.1
Database: emblrelpat Go to original record
Description: Sequence 10 from Patent EP1477557.
Taxon: synthetic construct
Original size: 13737 bp
Patents: EP1477557 (lens, espacenet)

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Method: QL-CON-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the Cauliflower Mosaic Virus 35S promoter and the pat gene
Target:CaMV P-35S-pat

View other 205 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 11340 11450 - 111 bp
Amplicon: 111 bp 
1     aagttcattt catttggaga ggacagggta cccggggatc ctctagagtc
51    gacatgtctc cggagaggag accagttgag attaggccag ctacagcagc
101   tgatatggcc g

View other 19 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 1.24e-11
primerB 100.0 21 bp 0 0 111 91 1.65e-09
probe 100.0 28 bp 0 0 60 87 2.89e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.