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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MI826470.1
Database: emblrelpat Go to original record
Description: Sequence 3 from patent US 9994863.
Taxon: unidentified
Original size: 5092 bp
Patents: US9994863 (lens, espacenet)
Method: QT-EVE-ZM-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event VCO-01981-5 (EURL GMFF, 2016).
Target:VCO-01981-5
Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 672 756 + 85 bp
Amplicon: 85 bp 
1     ccactgaacg tcaccaagaa gatcagtact caaacactga tagtttaaac
51    tgaagaagct taatttaaat ccctcgagta gcggc
Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.69e-10
primerB 100.0 20 bp 0 0 85 66 4.21e-09
probe 100.0 20 bp 0 0 24 43 4.21e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.