Match details

Record ID: MI638669.1
Database: emblrelpat Go to original record
Description: Sequence 1 from patent US 9961848.
Taxon: unidentified
Original size: 10656 bp
Patents: US9961848 (lens, espacenet)

Method: QT-EVE-GM-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event CV127 (Savini et al., 2011)
Target:CV127

View other 5 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6030 6117 + 88 bp

Amplicon: 88 bp
1     aacagaagtt tccgttgagc tttaagacgt ttggggaagc tgtcccatgc
51    ccatcaaaga agacagtaca cgatccgagc tacgaatg 

View other 5 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 2.39e-13
primerB 100.0 23 bp 0 0 88 66 1.13e-10
probe 100.0 23 bp 0 0 30 52 1.13e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.