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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KH945883.1
Database: emblrelpat Go to original record
Description: Sequence 7 from patent US 9719145.
Taxon: unidentified
Original size: 1626 bp
Patents: US9719145 (lens, espacenet)
Method: QT-EVE-GM-016 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event MON87751 (EURL GMFF, 2016).
Target:MON-87751-7

View other 11 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1280 1366 + 87 bp
Amplicon: 87 bp 
1     ctaaattgct ctttggagtt tattttgtag atatttcccc tcactttgga
51    gatctccagt cagcatcatc acaccaaaag ttaggcc

View other 11 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 30 bp 0 0 1 30 1.98e-14
primerB 100.0 24 bp 0 0 87 64 3.26e-11
probe 100.0 29 bp 0 0 62 34 6.85e-14
probe 100.0 12 bp 0 0 47 58 1.93e-04

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.