Match details

Record ID: KH197631.1
Database: emblrelpat Go to original record
Description: Sequence 6 from patent US 9447428.
Taxon: unidentified
Original size: 5946 bp
Patents: US9447428 (lens, espacenet)

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Method: QT-EVE-GM-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event MON87708 (Savini et al., 2013).
Target:MON87708

View other 13 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4094 4184 + 91 bp

Amplicon: 91 bp
1     tcatactcat tgctgatcca tgtagatttc ccggacttta gctcaaaatg
51    catgtattta ttagcgttct gtcttttcgt taatttgttc t 

View other 13 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 1.00e-11
primerB 100.0 27 bp 0 0 91 65 8.52e-13
probe 100.0 28 bp 0 0 29 56 2.48e-13


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.