Match details

Record ID:	HL913590.1
Database:	emblrelpat	Go to original record
Description:	Sequence 2 from patent US 9382550.
Taxon:	unidentified
Original size:	452 bp
Patents:	US9382550 (lens, espacenet)

Method:	QT-EVE-GH-009	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of cotton event T304-40 (Nardini et al., 2012).
Target:	T304-40

View other 4 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	80	+	78 bp

Amplicon:

78 bp

1     agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatctccttt
51    ttcttttcaa gttatcccaa gatctagg

View other 4 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	22 bp	0	0	1	22	3.36e-10
primerB	100.0	27 bp	0	0	78	52	7.23e-13
probe	100.0	23 bp	0	0	24	46	9.90e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.