Match details

Record ID: HL197268.1
Database: emblrelpat Go to original record
Description: Sequence 10 from patent US 9133473.
Taxon: unidentified
Original size: 1411 bp
Patents: US9133473 (lens, espacenet)

Method: QT-EVE-GH-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event MON531 (Mazzara et al., 2008).
Target:MON531

View other 5 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1060 1131 - 72 bp

Amplicon: 72 bp
1     aaccaatgcc accccactga cccacttagc agagaagaag tggagggaca
51    aacgtgagaa actcgaatgg ga 

View other 5 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.52e-09
primerB 100.0 21 bp 0 0 72 52 1.05e-09
probe 100.0 20 bp 0 0 51 32 3.52e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.