Match details

Record ID: HK513176.1
Database: emblrelpat Go to original record
Description: Sequence 2 from patent US 8993238.
Taxon: unidentified
Original size: 3104 bp
Patents: US8993238 (lens, espacenet)

Method: QT-EVE-BN-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event 73496 (Jacchia et al., 2014).
Target:DP-073496-4

View other 17 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 422 505 + 84 bp

Amplicon: 84 bp
1     gttcttctct tcatagctca ttacagtttt cattagttag atcaggatat
51    tcttgtttaa gatgttgaac tctatggagg tttg 

View other 17 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 30 bp 0 0 1 30 1.91e-14
primerB 100.0 28 bp 0 0 84 57 2.28e-13
probe 100.0 23 bp 0 0 33 55 1.07e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.