Match details
| Record ID: |
BD249858.1 |
| Database: |
emblrelpat |
Go to original record |
| Description: |
JP 2002536024-A/1: Test kit and method for quantitatively detecting genetically modi-fied DNA in foodstuff by means of fluorescence-coupled PCR. |
| Taxon: |
synthetic construct |
| Original size: |
240 bp |
| Patents: |
JP2002536024 (lens, espacenet)
|
View other 3 matches for this record
| Method: |
QT-CON-00-003 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of the junction between the CaMV35S promoter and the CTP sequence (ISO/FDIS 21570:2005). |
| Target: | CaMV P-35S-CTP |
View other 11 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
107 |
180 |
+ |
74 bp |
|
1 catttggaga ggacacgctg acaagctgac tctagcagat ctttcaagaa
51 tggcacaaat taacaacatg gctc
View other 2 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
1.08e-09 |
| primerB |
100.0 |
23 bp |
0 |
0 |
74 |
52 |
9.36e-11 |
| probe |
100.0 |
24 bp |
0 |
0 |
22 |
45 |
2.75e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
