Match details

Record ID:	GZ452839.1
Database:	emblrelpat	Go to original record
Description:	Sequence 59 from patent US 8232456.
Taxon:	unidentified
Original size:	1518 bp
Patents:	US8232456 (lens, espacenet)

View other 6 matches for this record

Method:	QT-EVE-ZM-022	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of maize event MIR162 (Charles Delobel et al., 2011)
Target:	MIR162

View other 17 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	265	356	+	92 bp

Amplicon:

92 bp

1     gcgcggtgtc atctatgtta ctagatcccc gggtctagac aattcagtac
51    attaaaaacg tccgccatgg tctgaaggca acagataagg ca

View other 17 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	24 bp	0	0	1	24	3.46e-11
primerB	100.0	22 bp	0	0	92	71	4.01e-10
probe	100.0	34 bp	0	0	34	67	1.39e-16

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.