Match details

Record ID:	GY225197.1
Database:	emblrelpat	Go to original record
Description:	Sequence 50 from patent US 7928296.
Taxon:	unidentified
Original size:	8112 bp
Patents:	US7928296 (lens, espacenet)

View other 6 matches for this record

Method:	QT-EVE-ZM-021	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of maize event 98140 (Savini et al., 2011)
Target:	DP-098140-6

View other 17 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	681	760	+	80 bp

Amplicon:

80 bp

1     gtgtgtatgt ctctttgctt ggtctttctc tatcgatccc cctctttgat
51    agtttaaact gaaggcggga aacgacaatc

View other 17 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	26 bp	0	0	1	26	2.55e-12
primerB	100.0	20 bp	0	0	80	61	3.94e-09
probe	100.0	33 bp	0	0	28	60	4.37e-16

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.