Match details

Record ID: GY044804.1
Database: emblrelpat Go to original record
Description: Sequence 3 from patent US 7897748.
Taxon: unidentified
Original size: 1312 bp
Patents: US7897748 (lens, espacenet)

Method: QT-EVE-ZM-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event MIR604 (Mazzara et al., 2007).
Target:MIR 604

View other 12 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 785 860 + 76 bp

Amplicon: 76 bp
1     gcgcacgcaa ttcaacagaa ggcgggaaac gacaatctga tcatgagcgg
51    agaattaagg gagtcacgtt atgacc 

View other 12 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.19e-08
primerB 100.0 26 bp 0 0 76 51 2.41e-12
probe 100.0 26 bp 0 0 20 45 2.41e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.