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Match details

Method:

QT-EVE-GH-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event LLCotton25 (Mazzara et al., 2007). Targets:5'-host genome<--->insert GMO-UID:ACS-GH001-3
View other 10 matches for this method


Target:

NRN_AX705314
Database:nrnl1 Go to original record
Description:Sequence 1 from Patent WO03013224. Sequence 1 from patent US 6818807. Sequence 1 from patent US 7442504. Sequence 1 from patent US 7834168.
Taxon:unidentified
Original size:850 bp
Patents: WO03013224 US6818807 US2003097687 US7442504 US2005005334 US7834168


Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 653 730 + 78


Amplicon:

78bp
1     caaggaacta ttcaactgag cttaacagta ctcggccgtc gaccgcggta
51    cccggaattc caatcccaca aaaatctg 
View other 8 matches with an identical amplicon


Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 20 0 0 1 20 3.84e-09
primerB 100.000 24 0 0 78 55 2.91e-11
probe 100.000 26 0 0 21 46 2.48e-12

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link


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