Navigation path

Additional tools

  • Print version
  • Decrease text
  • Increase text

Match details

Method:

QT-EVE-GH-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event LLCotton25 (Mazzara et al., 2007). Targets:5'-host genome<--->insert GMO-UID:ACS-GH001-3
View other 10 matches for this method


Target:

HQ233646.1
Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
Patents:
View other 15 matches for this record


Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 1698 1776 + 79


Amplicon:

79bp
1     caaggaacta ttcaactgag cttaacagta ctcggccgtc gaccgcggta
51    ccccggaatt ccaatcccac aaaaatctg 
View other 1 match with an identical amplicon


Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 20 0 0 1 20 3.89e-09
primerB 100.000 24 0 0 79 56 2.95e-11
probe 100.000 26 0 0 21 46 2.52e-12

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link


NEW! Download a How-To guide: "Using the EURL GMFF online bioinformatics resources"


© European Union, 2015

The information above is provided "as is" without warranty of any kind, either express or implied, including, but not limited to, fitness for a particular purpose and absence of errors in the application. The entire risk as to the use, quality, and performance of the application is with the user. The EU will not be liable for any incidental, consequential, direct or indirect damages including but not limited to the loss of data, loss of profits, or any other financial loss arising from the use or inability to use the application.