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Only one hit for query 'ac:MON-88913-8'
ID   QT-EVE-GH-007; SV 0; linear; genomic DNA; STS; SYN; 94 BP.
XX
AC   MON-88913-8;
XX
DT   03-APR-2009
DT   15-MAR-2012
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DE   Quantitative PCR method for detection of cotton event MON88913
DE   (Charles Delobel et al., 2009).
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KW   event_specific.
XX
OS   Gossypium hirsutum (upland cotton) - event MON88913 (MON-88913-8)
XX
RN   [1]
RP   1-94
RA   Charles Delobel C., Luque Perez E., Pinski G., Bogni A., Mazzara M., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Cotton MON 88913 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2009).
RX   DOI=10.2788/58818
XX
RN   [2]
RP   1-94
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-GH-007.pdf
XX
DR   GMOMETHODS; QT-TAX-GH-015;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..94
FT                   /standard_name="PCR 94 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of cotton event MON 88913 and the cotton host genome"
FT   primer_bind     1..28
FT                   /standard_name="Primer forward: MON88913 primer 2"
FT                   /note="CAAATTACCCATTAAGTAGCCAAATTAC"
FT                   /target="5'-host genome"
FT   primer_bind     complement(42..63)
FT                   /standard_name="RT-PCR probe: MON88913 probe"
FT                   /note="FAM-AACTATCAGTGTTTGACTACAT-MGBNFQ"
FT   primer_bind     complement(71..94)
FT                   /standard_name="Primer reverse: MON88913 primer 1"
FT                   /note="GGCTTTGGCTACCTTAAGAGAGTC"
FT                   /target="insert"
XX
SQ   Sequence 94 BP; 27 A; 17 C; 11 G; 19 T; 20 other;
     caaattaccc attaagtagc caaattacnn nnnnnnnnnn natgtagtca aacactgata        60
     gttnnnnnnn gactctctta aggtagccaa agcc                                    94
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