ID QT-TAX-ZM-001; SV 0; linear; genomic DNA; STS; PLN; 135 BP. XX AC ; XX DT 29-MAY-2009 DT 13-MAR-2013 XX DE Quantitative PCR method for detection of maize alcoholdeydrogenase 1 gene XX KW taxon_specific, validated_independently. XX OS Zea mays (maize) XX RN [1] RP 1-135 RT "Foodstuffs - Methods of analysis for the detection of genetically RT modified organisms and derived products - Quantitative nucleic acid based RT methods"; RL ISO 21570:1-103 (2005). RX ISO=34615 XX RN [2] RP 1-135 RA Mazzara M., Puumalaainen J., Van Den Eede G.; RT "Validation of the GMO Specific Detection Method Developed by NVI/INRA for Bt11 in Sweet Corn Maize - Validation Report and Protocol"; RL Online Publication (2005). RX BSHOP=LBNA21829 XX RN [3] RP 1-135 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2010). RX PCR=QT-TAX-ZM-001.pdf XX DR GMOMETHODS; QT-EVE-ZM-006; XX FH Key Location/Qualifiers FH FT STS 1..135 FT /standard_name="PCR 135 bp amplicon" FT /note="taxon-specific RT-PCR for maize" FT /target="alcohol deydrogenase 1 (adh1) gene" FT primer_bind 1..23 FT /standard_name="Primer forward: ADH-FF3" FT /note="CGTCGTTTCCCATCTCTTCCTCC" FT /target="adh1" FT primer_bind 44..70 FT /standard_name="RT-PCR probe: ADH1-MDO" FT /note="FAM-AATCAGGGCTCATTTTCTCGCTCCTCA-TAMRA" FT primer_bind complement(116..135) FT /standard_name="Primer reverse: ADH-RR4" FT /note="CCACTCCGAGACCCTCAGTC" FT /target="adh1" XX SQ Sequence 135 BP; 23 A; 36 C; 32 G; 44 T; 0 other; cgtcgtttcc catctcttcc tccnnnnnnn nnnnnnnnnn nnnaatcagg gctcattttc 60 tcgctcctca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactg 120 agggtctcgg agtgg 135 //