ID QL-ELE-00-003; SV 0; linear; genomic DNA; STS; SYN; 173 BP. XX AC ; XX DT 23-JUN-2009 DT 11-JAN-2018 XX DE Qualitative PCR method for detection of Neomycin phosphotransferase II (nptII) gene DE (BVL L 00.00-31, 1998) XX KW element_specific. XX RN [1] RP 1-173 RA Collection of Official Methods under Article 35 of the German Federal Foods Act; RT "Screening procedure for the detection of genetically modified DNA RT sequences in foods by identification of DNA sequences that frequently RT occur in genetically modified organisms"; RL Food Analysis, L 00.00-31, Beuth, Berlin Koln (1998). XX RN [2] RP 1-173 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2010). RX PCR=QL-ELE-00-003.pdf XX FH Key Location/Qualifiers FH FT STS 1..173 FT /standard_name="PCR 173 bp amplicon" FT /note="element-specific" FT /target="Neomycin phosphotransferase II (nptII) gene" FT primer_bind 1..16 FT /standard_name="Primer forward: TN5-1" FT /note="GGATCTCCTGTCATCT" FT /target="nptII" FT primer_bind complement(158..173) FT /standard_name="Primer reverse: TN5-2" FT /note="GATCATCCTGATCGAC" FT /target="nptII" XX SQ Sequence 173 BP; 6 A; 8 C; 8 G; 10 T; 141 other; ggatctcctg tcatctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gatcaggatg atc 173 //