ID QT-EVE-BN-014; SV 0; linear; genomic DNA; STD; PLN; 89 BP. XX AC MON-94100-2; XX DT 30-AUG-2022 DT 30-AUG-2022 XX DE Quantitative PCR method for detection of oilseed rape event MON94100 (EURL GMFF, 2022). XX KW event_specific. XX OS Brassica napus (rape) - event MON94100 (MON-94100-2) XX RN [1] RP 1-89 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Oilseed Rape MON 94100 RT Using Real-time PCR - Validation Report"; RL Online Publication (2022). RX EURL_GMFF=EURL-VL-04-20-VR.pdf RX EURL_GMFF=EURL-VL-04-20-VM.pdf XX RN [2] RP 1-89 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2022). RX PCR=QT-EVE-BN-014.pdf XX DR GMOMETHODS; QT-TAX-BN-001; XX FH Key Location/Qualifiers FH FT STS 1..89 FT /note="Event-specific RT-PCR" FT /target="5' integration border region (IBR) between the insert of oilseed rape event MON94100 and the oilseed rape host genome" FT primer_bind 1..31 FT /standard_name="Primer forward: MON 94100 primer 1" FT /note="CACCATCTAATGAATAGTCACCAAAATAACG" FT /target="5'-host genome" FT primer_bind complement(45..63) FT /standard_name="RT-PCR probe: MON 94100 probe" FT /note="FAM-TGATGCTGACTGGTGTCAA-MGBNFQ" FT primer_bind complement(65..89) FT /standard_name="Primer reverse: MON 94100 primer 2" FT /note="CTATTCGGGCCTAACTTTTGGTGTG" FT /target="insert" XX SQ Sequence 89 BP; 33 A; 24 C; 13 G; 19 T; 0 other; caccatctaa tgaatagtca ccaaaataac gnnnnnnnnn nnnnttgaca ccagtcagca 60 tcancacacc aaaagttagg cccgaatag 89 //