ID QT-TAX-OS-002; SV 0; linear; mRNA; STD; PLN; 124 BP. XX AC ; XX DT 09-JUN-2008 DT 08-APR-2016 XX DE Quantitative PCR method for detection of rice phospholipase D alpha 2 gene (Jacchia et al., 2015) XX KW Taxon_specific , validated_in_combination. XX OS Oryza sativa (rice) XX RN [1] RP 1-124 RA Jacchia S., Nardini E., Bassani N., Savini C., Shim J-H., Trijatmiko K., Kreysa J., Mazzara M.; RT " International ring trial for the validation of an event-specific Golden Rice 2 quantitative real-time polymerase chain reaction method"; RL J. Agric. Food Chem. 63:4954-4965 (2015). RX DOI=10.1021/acs.jafc.5b00951 XX RN [2] RP 1-124 RA Jacchia S., Nardini E., Savini C., Petrillo M., Angers-Loustau A., Shim J-H., Trijatmiko K., Kreysa J., Mazzara M.; RT " Development, optimization, and single laboratory validation of an event-specific real-time PCR method for the detection and quantification of Golden Rice 2 using a novel taxon-specific assay"; RL J. Agric. Food Chem. 63:1711-1721 (2015). RX DOI=10.1021/jf505516y XX RN [3] RP 1-124 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2016). RX PCR=QT-TAX-OS-002.pdf XX DR GMOMETHODS; QT-EVE-OS-001; XX FH Key Location/Qualifiers FH FT STS 1..124 FT /standard_name="PCR 124 bp amplicon" FT /note="taxon-specific RT-PCR for rice" FT /target="phospholipase D alpha 2 (PLD) gene" FT primer_bind 1..22 FT /standard_name="Primer forward: PLD-GR For" FT /note="GGCAAGAACAACACCATTGACA" FT /target="PLD ?2" FT primer_bind 52..73 FT /standard_name="RT-PCR probe: PLD-GR Probe" FT /note="FAM-ATTCGCCGCGCCAAGAACTTCA-TAMRA" FT primer_bind complement(106..124) FT /standard_name="Primer reverse: PLD-GR Rev" FT /note="CGGCTTTCCATGCAAATGA" FT /target="PLD ?2" XX SQ Sequence 124 BP; 39 A; 36 C; 25 G; 24 T; 0 other; ggcaagaaca acaccattga cannnnnnnn nnnnnnnnnn nnnnnnnnnn nattcgccgc 60 gccaagaact tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcatt tgcatggaaa 120 gccg 124 //