ID QT-TAX-ZM-014; SV 0; linear; genomic DNA; STS; PLN; 135 BP. XX AC ; XX DT 29-MAY-2009 DT 18-JAN-2021 XX DE Quantitative PCR method for detection of maize alcoholdeydrogenase 1 gene XX KW taxon_specific, validated_in_combination. XX OS Zea mays (maize) XX RN [1] RP 1-135 RA Mazzara M., Munaro B., Foti N., Savini C., Van Den Eede G.; RT "Event-specific Method for the Quantification of Maize Line MIR604 Using Real-time PCR - Validation Report and Protocol - Maize Seeds Sampling and DNA Extraction"; RL Online Publication (2007). RX DOI=10.2788/30596 XX RN [2] RP 1-135 RA Charles Delobel C., Foti N., Mazzara M., Van Den Eede G.; RT "Event-specific Method for the Quantification of Maize Event 3272 Using Real-time PCR - Validation Report and Protocol"; RL Online Publication (2008). RX DOI=10.2788/43051 XX RN [3] RP 1-135 RA Charles Delobel C., Larcher S., Mazzara M., Van Den Eede G.; RT "Event-specific Method for the Quantification of Maize Event Bt11 Using Real-time PCR - Validation Report and Protocol"; RL Online Publication (2008). RX DOI=10.2788/4370 XX RN [4] RP 1-135 RA Charles Delobel C., Larcher S., Savini C., Mazzara M., Van Den Eede G.; RT "Event-specific Method for the Quantification of Maize Line GA21 Using Real-time PCR - Validation Report and Protocol - Report on the Verification of Performance of a DNA Extraction Method for Maize Grains"; RL Online Publication (2007). RX DOI=10.2788/62889 XX RN [5] RP 1-135 RA Charles Delobel C., Mazzara M., Bevilacqua A., Van den Eede G.; RT "Event-specific Method for the Quantification of Maize MIR162 by Real-time PCR - Validation Report and Protocol"; RL Online Publication (2011). RX DOI=10.2788/44161 XX RN [6] RP 1-135 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "In-house Validation of an Event-specific Method for the Quantification of Maize Bt176 Using Real-time PCR - Validation Report"; RL Online Publication (2011). RX EURL_GMFF=Bt176_CRLVL1804_VR.pdf RX EURL_GMFF=Bt176_CRLVL1804_validated_Method.pdf XX RN [7] RP 1-135 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Maize 5307 by Real-time PCR - Validation Report and Validated Method"; RL Online Publication (2014). RX EURL_GMFF=EURL-VL-07-11VR_Maize 5307 final report.pdf RX EURL_GMFF=2014-12-05_VP_EURL-VL-07-11FINAL.pdf XX RN [8] RP 1-135 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Maize MZHG0JG Using Real-time PCR - Validation Report"; RL Online Publication (2018). RX EURL_GMFF=EURL-VL-04-16-VM.pdf RX EURL_GMFF=EURL-VL-04-16-VR.pdf XX RN [9] RP 1-135 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Maize MZIR098 Using Real-time PCR - Validation Report"; RL Online Publication (2018). RX EURL_GMFF=EURL-VL-04-17-VR.pdf RX EURL_GMFF=EURL-VL-04-17-VM.pdf XX RN [10] RP 1-135 RT "See Cross-references below"; RL Online Publication (2010). XX DR GMOMETHODS; QT-EVE-ZM-013; DR GMOMETHODS; QT-EVE-ZM-019; DR GMOMETHODS; QT-EVE-ZM-015; DR GMOMETHODS; QT-EVE-ZM-014; DR GMOMETHODS; QT-EVE-ZM-022; DR GMOMETHODS; QT-EVE-ZM-023; DR GMOMETHODS; QT-EVE-ZM-002; DR GMOMETHODS; QT-EVE-ZM-027; DR GMOMETHODS; QT-EVE-ZM-028; XX FH Key Location/Qualifiers FH FT STS 1..135 FT /standard_name="PCR 135 bp amplicon" FT /note="taxon-specific RT-PCR for maize" FT /target="alcohol deydrogenase 1 (adh1) gene" FT primer_bind 1..23 FT /standard_name="Primer forward: Zm Adh1 primer F" FT /note="CGTCGTTTCCCATCTCTTCCTCC" FT /target="adh1" FT primer_bind 44..70 FT /standard_name="RT-PCR probe: Zm Adh1" FT /note="VIC-AATCAGGGCTCATTTTCTCGCTCCTCA-TAMRA" FT primer_bind complement(116..135) FT /standard_name="Primer reverse: Zm Adh1 primer R" FT /note="CCACTCCGAGACCCTCAGTC" FT /target="adh1" XX SQ Sequence 135 BP; 23 A; 36 C; 32 G; 44 T; 0 other; cgtcgtttcc catctcttcc tccnnnnnnn nnnnnnnnnn nnnaatcagg gctcattttc 60 tcgctcctca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactg 120 agggtctcgg agtgg 135 //