ID QT-EVE-GM-010; SV 0; linear; genomic DNA; STS; SYN; 89 BP. XX AC MON-87701-2; XX DT 04-AUG-2011 DT 26-AGO-2011 XX DE Quantitative PCR method for detection of soybean event MON87701 DE (Charels et al., 2011) XX KW event_specific. XX OS Glycine max (soybean) - event MON87701 (MON-87701-2) XX RN [1] RP 1-89 RA Charels D. et al.,; RT "Event-specific Method for the Quantification of Soybean MON87701 Using Real-time PCR - Validation Report and Protocol"; RL Online Publication (2011). RX EURL_GMFF=2011-01-18_MON 87701_EURLVL0509_validated_Method.pdf RX EURL_GMFF=2010-07-28_MON87701VR_EURLVL0509.pdf XX RN [2] RP 1-89 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2011). RX PCR=QT-EVE-GM-010.pdf XX DR GMOMETHODS; QT-TAX-GM-002; XX FH Key Location/Qualifiers FH FT STS 1..89 FT /standard_name="PCR 89 bp amplicon" FT /note="event-specific RT-PCR" FT /target="5' integration border region (IBR) between the insert of soybean event MON87701 and the soybean host genome"; FT primer_bind 1..28 FT /standard_name="Primer forward: MON 87701 primer 2" FT /note="TGGTGATATGAAGATACATGCTTAGCAT" FT /target="5'-host genome" FT primer_bind complement(35..64) FT /standard_name="RT-PCR probe: MON 87701 probe" FT /note="FAM-TCAGTGTTTGACACACACACTAAGCGTGCC-TAMRA" FT primer_bind complement(68..89) FT /standard_name="Primer reverse: MON 87701 primer 1" FT /note="CGTTTCCCGCCTTCAGTTTAAA" FT /target="insert" XX SQ Sequence 89 BP; 26 A; 16 C; 25 G; 22 T; 0 other; tggtgatatg aagatacatg cttagcatnn nnnnggcacg cttagtgtgt gtgtcaaaca 60 ctgannnttt aaactgaagg cgggaaacg 89 //