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EU Database of Reference Methods for GMO Analysis
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Only one hit for query 'ac:MON-88017-3'
Entry information
Entry name QT-EVE-ZM-016;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier MON-88017-3
Description Quantitative PCR method for detection of maize event MON88017 (Charles Delobel et al., 2008).
Keywords event_specific.
From Zea mays (maize) - event MON88017 (MON-88017-3)
1 Charles Delobel C., Foti N., Grazioli E., Mazzara M., Van Den Eede G.; "Event-specific Method for the Quantification of Maize Line MON 88017 Using Real-time PCR - Validation Report and Protocol" Online Publication (2008)
DOI 10.2788/43272
Reference Position 1-95
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QT-EVE-ZM-016.pdf
Reference Position 1-95
Key Location Qualifier Value
STS 1..95 standard_namePCR 95 bp amplicon
noteevent-specific RT-PCR
target3' integration border region (IBR) between the insert of maize event MON 88017 and the maize host genome
primer_b 1..20 standard_namePrimer forward: MON88017 AF
primer_b 47..75 standard_nameRT-PCR probe: MON88017 AP
primer_b complement(77..95) standard_namePrimer reverse: MON88017 AR
target3'-host genome
misc_dif 94 noteThere is a mismatch between the sequence 'T' of the rev-primer MON88017 Primer 2 and the reported genomic sequence 'C'of MON88017. According to Monsanto this does not have a bad influence on the PCR and the method has been validated.
Sequence information
Length: 95 BP, A Count: 16, C Count: 18, T Count: 14, G Count: 20
gagcaggacc tgcagaagct nnnnnnnnnn nnnnnnnnnn nnnnnntccc gccttcagtt        60
taaacagagt cgggtntgga tggtcaactc cggca                                   95