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EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Only one hit for query 'ac:DAS-59122-7'
Entry information
Entry name QT-EVE-ZM-012;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier DAS-59122-7
Description Quantitative PCR method for detection of maize event 59122 (Mazzara et al., 2006).
Keywords event_specific.
From Zea mays (maize) - event 59122 (DAS-59122-7)
1 Mazzara M., Grazioli E., Larcher S., Savini C., Van Den Eede G.; "Event-Specific Method for the Quantitation of Maize Line DAS-59122-7 Using Real-Time PCR - Validation Report and Protocol" Online Publication (2006)
DOI 10.2788/31820
Reference Position 1-86
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QT-EVE-ZM-012.pdf
Reference Position 1-86
Key Location Qualifier Value
STS 1..86 standard_namePCR 86 bp amplicon
noteevent-specific RT-PCR
targetIntegration border region (IBR) between the insert of maize event 59122 and the maize host genome
primer_b 1..23 standard_namePrimer forward: DAS-59122-7-rb1f
targetnot specified
primer_b 36..61 standard_nameRT-PCR probe: DAS-59122-7-rb1s
primer_b complement(63..86) standard_namePrimer reverse: DAS-59122-rb1r
targetnot specified
Sequence information
Length: 86 BP, A Count: 28, C Count: 11, T Count: 12, G Count: 22
gggataagca agtaaaagcg ctcnnnnnnn nnnnntttaa actgaaggcg ggaaacgaca        60
anctgatcat gagcggagaa ttaagg                                             86