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GMOMETHODS:
EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Only one hit for query 'ac:DAS-01507-1'
Entry information
Entry name QT-EVE-ZM-010;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier DAS-01507-1
Description
Description Quantitative PCR method for detection of maize event TC1507 (Mazzara et al., 2005).
Keywords event_specific.
From Zea mays (maize) - event TC1507 (DAS-01507-1)
References
1 Mazzara M., Foti N., Price S., Paoletti C., Van Den Eede G.; "Event-Specific Method for the Quantitation of Maize Line TC1507 Using Real-Time PCR - Validation Report and Protocol - Sampling and DNA Extraction of Maize TC15O7" Online Publication (2005)
BSHOP LBNA21828
Reference Position 1-58
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QT-EVE-ZM-010.pdf
Reference Position 1-58
Cross-references
GMOMETHODS QT-TAX-ZM-002;
Features
Key Location Qualifier Value
STS 1..58 standard_namePCR 58 bp amplicon
noteevent-specific RT-PCR
targetintegration border region (IBR) between the insert of maize event TC1507 and the maize host genome
primer_b 1..19 standard_namePrimer forward: MaiY-F1
noteTAGTCTTCGGCCAGAATGG
targetnot specified
primer_b 22..42 standard_nameRT-PCR probe: MaiY-S1
noteFAM-TAACTCAAGGCCCTCACTCCG-TAMRA
primer_b complement(41..58) standard_namePrimer reverse: Maiy-R3
noteCTTTGCCAAGATCAAGCG
targetnot specified
Sequence information
Length: 58 BP, A Count: 13, C Count: 18, T Count: 14, G Count: 13
tagtcttcgg ccagaatggn ntaactcaag gccctcactc cgcttgatct tggcaaag          58