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ID   QT-EVE-ZM-007; SV 0; linear; genomic DNA; STS; SYN; 112 BP.
XX
AC   MON-00021-9;
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DT   26-JAN-2006
DT   14-OCT-2010
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DE   Quantitative PCR method for detection of maize event GA21
DE   (Paoletti et al., 2005).
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KW   event_specific.
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OS   Zea mays (maize) - event GA21 (MON-00021-9)
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RN   [1]
RP   1-112
RA   Paoletti C., Mazzara M., Puumalaainen J., Rasulo D., Van Den Eede G.;
RT   "Validation of an Event-Specific Method for the Quantitation of Maize Line GA21 Using Real-Time PCR Validation Report and Protocol";
RL   Online Publication (2005).
RX   CRL=GA21_Validation_Report+Protocol[1].pdf
XX
RN   [2]
RP   1-112
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-ZM-007.pdf
XX
DR   GMOMETHODS; QT-TAX-ZM-011;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..112
FT                   /standard_name="PCR 112 bp amplicon (Monsanto)"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event GA21 and the maize host genome"
FT   primer_bind     1..29
FT                   /standard_name="Primer forward: GA21 primer F"
FT                   /note="CTTATCGTTATGCTATTTGCAACTTTAGA"
FT                   /target="5'-host genome"
FT   primer_bind     31..70
FT                   /standard_name="RT-PCR probe: GA21 Probe PR"
FT                   /note="FAM-CATATACTAACTCATATCTCTTTCTCAACAGCAGGTGGGT-
FT                   TAMRA"
FT   primer_bind     complement(96..112)
FT                   /standard_name="Primer reverse: GA21 primer R"
FT                   /note="TGGCTCGCGATCCTCCT"
FT                   /target="insert"
XX
SQ   Sequence 112 BP; 23 A; 19 C; 17 G; 27 T; 26 other;
     cttatcgtta tgctatttgc aactttagan catatactaa ctcatatctc tttctcaaca        60
     gcaggtgggt nnnnnnnnnn nnnnnnnnnn nnnnnaggag gatcgcgagc ca               112
//