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ID   QT-EVE-OS-001; SV 0; linear; genomic DNA; STS; SYN; 121 BP.
XX
AC   ;
XX
DT   17-JUN-2013
DT   08-APR-2016
XX
DE   Quantitative PCR method for detection of rice event Golden Rice 2 (Jacchia et al., 2015)
XX
KW   event_specific.
XX
OS   Oryza sativa (rice) - event Golden Rice 2 (GR2)
XX
RN   [1]
RP   1-121
RA   Jacchia S., Nardini E., Bassani N., Savini C., Shim J-H., Trijatmiko K., Kreysa J., Mazzara M.;
RT   " International ring trial for the validation of an event-specific Golden Rice 2 quantitative real-time polymerase chain reaction method";
RL   J. Agric. Food Chem. 63:4954-4965 (2015).
RX   DOI=10.1021/acs.jafc.5b00951
XX
RN   [2]
RP   1-121
RA   Jacchia S., Nardini E., Savini C., Petrillo M., Angers-Loustau A., Shim J-H., Trijatmiko K., Kreysa J., Mazzara M.;
RT   " Development, optimization, and single laboratory validation of an event-specific real-time PCR method for the detection and quantification of Golden Rice 2 using a novel taxon-specific assay";
RL   J. Agric. Food Chem. 63:1711-1721 (2015).
RX   DOI=10.1021/jf505516y
XX
RN   [3]
RP   1-121
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QT-EVE-OS-001.pdf
XX
DR   GMOMETHODS; QT-TAX-OS-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..121
FT                   /standard_name="PCR 121 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of rice event Golden Rice 2 and the rice host genome"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: GR2 For"
FT                   /note="TGGCCGTATCCGCAATGTG"
FT                   /target="insert"
FT   primer_bind     33..59
FT                   /standard_name="RT-PCR probe: GR2 Probe"
FT                   /note="FAM-TCGATATGATTCCTTCATCGAGCGAGC-TAMRA"
FT   primer_bind     complement(101..121)
FT                   /standard_name="Primer reverse: RB-REV-BS"
FT                   /note="GCTGCGCCTCTAACCAGGTAT"
FT                   /target="3'-host genome"
XX
SQ   Sequence 121 BP; 24 A; 27 C; 39 G; 31 T; 0 other;
     tggccgtatc cgcaatgtgn nnnnnnnnnn nntcgatatg attccttcat cgagcgagcn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atacctggtt agaggcgcag       120
     c                                                                       121
//