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GMOMETHODS:
EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Only one hit for query 'ac:DAS-21023-5'
Entry information
Entry name QT-EVE-GH-001b;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier DAS-21023-5
Description
Description Quantitative PCR method for detection of cotton event 3006-210-23 (Mazzara et al., 2006).
Keywords event_specific.
From Gossypium hirsutum (upland cotton) - event 3006-210-23 (DAS-21023-5)
References
1 Mazzara M., Larcher S., Savini C., Charles Delobel C., Van Den Eede G.; "Event-Specific Methods for the Quantitation of the Hybrid Cotton Line 281-24-236/3006-210-23 Using Real-Time PCR - Validation Report and Protocol - Sampling and DNA Extraction of Cotton Seeds" Online Publication (2006)
DOI 10.2788/32649
Reference Position 1-90
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QT-EVE-GH-001b.pdf
Reference Position 1-90
Cross-references
GMOMETHODS QT-TAX-GH-016;
QT-TAX-GH-021;
Features
Key Location Qualifier Value
STS 1..90 standard_namePCR 90 bp amplicon
noteevent-specific RT-PCR
target5' integration border region (IBR) between the insert of cotton event 3006-210-23 and the cotton host genome
primer_b 1..27 standard_namePrimer forward: 3006-f3
noteAAATATTAACAATGCATTGAGTATGATG
target5'-host genome
primer_b complement(31..60) standard_nameRT-PCR probe: 3006-s2
noteFAM- TACTCATTGCTGATCCATGTAGATTTCCCG-TAMRA
primer_b complement(65..90) standard_namePrimer reverse: 3006-r2
noteACTCTTTCTTTTTCTCCATATTGACC
targetinsert
Sequence information
Length: 90 BP, A Count: 36, C Count: 8, T Count: 19, G Count: 20
aaatattaac aatgcattga gtatgatgnn cgggaaatct acatggatca gcaatgagta        60
nnnnggtcaa tatggagaaa aagaaagagt                                         90