Perform your search by keyword, select a GMO unique identifier or click a link in the section below.


ID   QT-EVE-BN-005; SV 0; linear; genomic DNA; STS; SYN; 187 BP.
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AC   ACS-BN004-7;
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DT   22-NOV-2011
DT   16-JAN-2008
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DE   Quantitative PCR method for detection of oilseed rape event Ms1 (verified by the EU-RL GMFF in the context of Commission Decision 2007/305/EC)
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KW   event_specific, EU-RL_GMFF_in-house_verified.
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OS   Brassica napus (oilseed rape) - event Ms1 (ACS-BN004-7)
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RN   [1]
RP   1-187
RA   Savini C., ;
RT   "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape MS1 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=CRLVL1104 VR.pdf
RX   EURL_GMFF=CRLVL1104 VP.pdf
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RN   [2]
RP   1-187
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2011).
RX   PCR=QT-EVE-BN-005.pdf
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DR   GMOMETHODS; QT-TAX-BN-012;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..187
FT                   /standard_name="PCR 187 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="3'integration border region (IBR) between the insert of oilseed rape event Ms1 and the oilseed rape host genome";
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: MLD025"
FT                   /note="ACGCTGCGGACATCTACATT"
FT                   /target="insert"
FT   primer_bind     79..105
FT                   /standard_name="RT-PCR probe: TM030"
FT                   /note="FAM-CTCATTGCTGATCCACCTAGCCGACTT-TAMRA"
FT   primer_bind     complement(166..187)
FT                   /standard_name="Primer reverse: MDB175"
FT                   /note="CTAGATCGGAAGCTGAAGATGG"
FT                   /target="3'-host genome"
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SQ   Sequence 187 BP; 53 A; 50 C; 21 G; 63 T; 0 other;
     acgctgcgga catctacatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnct cattgctgat ccacctagcc gacttnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatc ttcagcttcc       180
     gatctag                                                                 187
//