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ID   QT-CON-00-002; SV 0; linear; genomic DNA; STS; SYN; 88 BP.
XX
AC   MON-04032-6;
XX
DT   29-MAY-2009
DT   06-OCT-2016
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DE   Quantitative PCR method for detection of the junction between the CTP sequence and the CP4 epsps gene (Hird et al., 2003).
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KW   construct_specific.
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OS   Glycine max (soybean) - event GTS-40-3-2 (MON-04032-6)
XX
RN   [1]
RP   1-88
RA   Hird H., Powell J., Johnson M.L., Oehlschlager S.;
RT   "Determination of percentage of RoundUp Ready soya in soya flour using
RT   real-time polymerase chain reaction: interlaboratory study";
RL   J AOAC Int 86:66-71 (2003).
RX   PUBMED; 12607742.
XX
RN   [2]
RP   1-88
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-CON-00-002.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-004;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..88
FT                   /standard_name="PCR 88 bp amplicon"
FT                   /note="Construct-specific duplex RT-PCR"
FT                   /target="Junction region between the chloroplast transit peptide (CTP) sequence from Petunia hybrida epsps gene and the CP4 epsps gene from Agrobacterium tumefasciens"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: RR-F"
FT                   /note="GGATTTCAGCATCAGTGGCTACA"
FT                   /target="CTP4"
FT   primer_bind     complement(33..53)
FT                   /standard_name="RT-PCR probe: RR soya"
FT                   /note="FAM-CCGGCTGCTTGCACCGTGAAG-TAMRA"
FT   primer_bind     complement(71..88)
FT                   /standard_name="Primer reverse: RR-R"
FT                   /note="CCGGAAAGGCCAGAGGAT"
FT                   /target="CP4-EPSPS"
XX
SQ   Sequence 88 BP; 16 A; 32 C; 23 G; 17 T; 0 other;
     ggatttcagc atcagtggct acannnnnnn nncttcacgg tgcaagcagc cggnnnnnnn        60
     nnnnnnnnnn atcctctggc ctttccgg                                           88
//