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ID   QL-TAX-OS-003; SV 0; linear; genomic DNA; STS; PLN; 68 BP.
XX
AC   ;
XX
DT   04-AUG-2009
DT   26-FEB-2016
XX
DE   Qualitative PCR method for detection of rice root-specific GOS9 gene
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Oryza sativa (rice)
XX
RN   [1]
RP   1-68
RA   Grohmann L., Maede D.;
RT   "Detection of genetically modified rice: collaborative validation study
RT   of a construct-specific real-time PCR method for detection of transgenic
RT   Bt rice";
RL   Eur. Food Res. Technol. 228:497-500 (2009).
RX   DOI=10.1007/s00217-008-0964-1
XX
RN   [2]
RP   1-68
RA   Savini C., Querci M., Ermolli M., Mazzara M., Cordeil S., Van den Eede
RA   G.;
RT   "Report on the Verification of the Performance of a Method for the
RT   Detection of 'Bt63' Rice Using Real-Time PCR";
RL   Online Publication (2008).
RX   EURL_GMFF=Bt63_Rice_verification_report_final.pdf
XX
RN   [3]
RP   1-68
RA   Grohmann L., Reiting R., Maede D., Uhlig S., Simon K., Frost K.,
RA   Jit Randhawa G., Zur K.;
RT   "Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products";
RL   Accred Qual Assur 20:85-96 (2015).
RX   DOI=10.1007/s00769-015-1108-5
XX
RN   [4]
RP   1-68
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QL-CON-00-007;
DR   GMOMETHODS; QL-CON-00-009;
DR   GMOMETHODS; QL-CON-00-013;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..68
FT                   /standard_name="PCR 68 bp amplicon"
FT                   /note="Taxon-specific RT-PCR for rice"
FT                   /target="rice root-specific GOS9 gene"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: org1"
FT                   /note="TTAGCCTCCCGCTGCAGA"
FT                   /target="GOS9"
FT   primer_bind     20..43
FT                   /standard_name="RT-PCR probe: orgp"
FT                   /note="FAM-CGGCAGTGTGGTTGGTTTCTTCGG-Dabcyl"
FT   primer_bind     complement(49..68)
FT                   /standard_name="Primer reverse: org2"
FT                   /note="AGAGTCCACAAGTGCTCCCG"
FT                   /target="GOS9"
XX
SQ   Sequence 68 BP; 8 A; 18 C; 24 G; 18 T; 0 other;
     ttagcctccc gctgcaganc ggcagtgtgg ttggtttctt cggnnnnncg ggagcacttg        60
     tggactct                                                                 68
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