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GMOMETHODS:
EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Only one hit for query 'id:QL-ELE*&ft:CP4'
Entry information
Entry name QL-ELE-00-019;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier
Description
Description Qualitative PCR method for detection of CP4 epsps gene (Barbau-Piednoir et al., 2014).
Keywords element_specific.
1 Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G., Van den Bulcke M.; "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR Screening(CoSYPS)" Food Analitical Methods 0:0-0 (2014)
DOI 10.1007/s12161-014-9837-3
Reference Position 1-108
2 "PCR reactions set up and amplification conditions" Online Publication (2014)
PCR QL-ELE-00-019.pdf
Reference Position 1-108
Cross-references
GMOMETHODS QL-TAX-BN-003;
QL-TAX-GM-001;
QL-TAX-ZM-002;
QL-PLN-00-006;
Features
Key Location Qualifier Value
STS 1..108 standard_namePCR 108 bp amplicon
noteelement-specific duplex PCR
targetCP4 epsps gene from Agrobacterium tumefasciens type I and II
primer_b 1..18 standard_namePrimer forward: CP4 Synthetic F
noteGCATGCTTCACGGTGCAA
targetCP4-EPSPS I
primer_b complement(90..108) standard_namePrimer reverse: CP4 Synthetic R
noteTGAAGGACCGGTGGGAGAT
targetCP4-EPSPS II
primer_b complement(90..108) standard_namePrimer reverse: CP4 synth Rbis
noteTGAAGGACCTGTGGGAGAT
targetCP4-EPSPS
Sequence information
Length: 108 BP, A Count: 19, C Count: 45, T Count: 19, G Count: 25
gcatgcttca cggtgcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctcccaccg gtccttca                    108