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ID   QT-TAX-ZM-001; SV 0; linear; genomic DNA; STS; PLN; 135 BP.
XX
AC   ;
XX
DT   29-MAY-2009
DT   13-MAR-2013
XX
DE   Quantitative PCR method for detection of maize alcoholdeydrogenase 1 gene
XX
KW   taxon_specific, validated_independently.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-135
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [2]
RP   1-135
RA   Mazzara M., Puumalaainen J., Van Den Eede G.;
RT   "Validation of the GMO Specific Detection Method Developed by NVI/INRA for Bt11 in Sweet Corn Maize - Validation Report and Protocol";
RL   Online Publication (2005).
RX   BSHOP=LBNA21829
XX
RN   [3]
RP   1-135
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-TAX-ZM-001.pdf
XX
DR   GMOMETHODS; QT-EVE-ZM-006;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..135
FT                   /standard_name="PCR 135 bp amplicon"
FT                   /note="taxon-specific RT-PCR for maize"
FT                   /target="alcohol deydrogenase 1 (adh1) gene"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: ADH-FF3"
FT                   /note="CGTCGTTTCCCATCTCTTCCTCC"
FT                   /target="adh1"
FT   primer_bind     44..70
FT                   /standard_name="RT-PCR probe: ADH1-MDO"
FT                   /note="FAM-AATCAGGGCTCATTTTCTCGCTCCTCA-TAMRA"
FT   primer_bind     complement(116..135)
FT                   /standard_name="Primer reverse: ADH-RR4"
FT                   /note="CCACTCCGAGACCCTCAGTC"
FT                   /target="adh1"
XX
SQ   Sequence 135 BP; 23 A; 36 C; 32 G; 44 T; 0 other;
     cgtcgtttcc catctcttcc tccnnnnnnn nnnnnnnnnn nnnaatcagg gctcattttc        60
     tcgctcctca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactg       120
     agggtctcgg agtgg                                                        135
//