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ID   QT-TAX-OS-017; SV 0; linear; mRNA; STS; PLN; 68 BP.
XX
AC   ;
XX
DT   09-JUN-2008
DT   17-NOV-2010
XX
DE   Quantitative PCR method for detection of rice Phospholipase D alpha 2 gene
DE   (Mazzara et al., 2006).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Oryza sativa Japonica Group (rice)
XX
RN   [1]
RP   1-68
RA   Mazzara M., Grazioli E., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantitation of Rice Line LLRICE62 Using Real-time PCR -Validation Report and Protocol - Sampling and DNA Extraction of Rice";
RL   Online Publication (2006).
RX   DOI=10.2788/31940
XX
RN   [2]
RP   1-68
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-EVE-OS-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..68
FT                   /gene="Os06g0604400"
FT                   /standard_name="PCR 68 bp amplicon"
FT                   /note="taxon-specific RT-PCR"
FT                   /target="phospholipase D (PLD) gene"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: KVM159"
FT                   /note="TGGTGAGCGTTTTGCAGTCT"
FT                   /target="PLD"
FT   primer_bind     22..44
FT                   /standard_name="RT-PCR probe: TM013"
FT                   /note="FAM-TGTTGTGCTGCCAATGTGGCCTG-TAMRA"
FT   primer_bind     complement(47..68)
FT                   /standard_name="Primer reverse: KVM160"
FT                   /note="CTGATCCACTAGCAGGAGGTCC"
FT                   /target="PLD"
XX
SQ   Sequence 68 BP; 8 A; 14 C; 22 G; 21 T; 3 other;
     tggtgagcgt tttgcagtct ntgttgtgct gccaatgtgg cctgnnggac ctcctgctag        60
     tggatcag                                                                 68
//