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GMOMETHODS:
EU Database of Reference Methods for GMO Analysis
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Entry information
Entry name QL-ELE-00-014;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier
Description
Description Qualitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Grohmann et al., 2009).
Keywords element_specific.
1 Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.; "Collaborative trial validation studies of real-time PCR-based GMO screening methods for detection of the bar gene and the ctp2-cp4epsps construct" J. Agric. Food Chem. 57:8913-8920 (2009)
PUBMED 19807158
DOI 10.1021/jf901598r
Reference Position 1-60
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QL-ELE-00-014.pdf
Reference Position 1-60
Features
Key Location Qualifier Value
STS 1..60 standard_namePCR 60 bp amplicon
noteelement-specific RT-PCR
targetphosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus
primer_b 1..19 standard_namePrimer forward: RapB-F1
noteACAAGCACGGTCAACTTCC
targetbar
primer_b 20..42 standard_nameRT-PCR probe: RapB-S1
noteFAM-TACCGAGCCGCAGGAACC-TAMRA
primer_b complement(43..60) standard_namePrimer reverse: RapB-R1
noteGAGGTCGTCCGTCCACTC
targetbar
Sequence information
Length: 60 BP, A Count: 10, C Count: 12, T Count: 5, G Count: 10
acaagcacgg tcaacttccn nnnnnnnnnn nnnnnnnnnn nngagtggac ggacgacctc        60