| Entry information |
|---|
| Entry name | QT-EVE-ZM-007; See in JRC GMO-Matrix See in JRC GMO-Amplicons |
| GMO Unique Identifier | MON-00021-9 |
| Description |
|---|
| Description | Quantitative PCR method for detection of maize event GA21 (Paoletti et al., 2005). |
| Keywords | event_specific. |
| From | Zea mays (maize) - event GA21 (MON-00021-9) |
| References |
|---|
| 1 | Paoletti C., Mazzara M., Puumalaainen J., Rasulo D., Van Den Eede G.; "Validation of an Event-Specific Method for the Quantitation of Maize Line GA21 Using Real-Time PCR Validation Report and Protocol" Online Publication (2005)
|
| 2 | "PCR reactions set up and amplification conditions" Online Publication (2010)
|
| Cross-references |
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| GMOMETHODS | QT-TAX-ZM-011; |
| Features |
|---|
| Key | Location | Qualifier | Value |
|---|
| STS | 1..112 | standard_name | PCR 112 bp amplicon (Monsanto) | | note | event-specific RT-PCR | | target | 5' integration border region (IBR) between the insert of maize event GA21 and the maize host genome | | primer_b | 1..29 | standard_name | Primer forward: GA21 primer F | | note | CTTATCGTTATGCTATTTGCAACTTTAGA | | target | 5'-host genome | | primer_b | 31..70 | standard_name | RT-PCR probe: GA21 Probe PR | | note | FAM-CATATACTAACTCATATCTCTTTCTCAACAGCAGGTGGGT- TAMRA | | primer_b | complement(96..112) | standard_name | Primer reverse: GA21 primer R | | note | TGGCTCGCGATCCTCCT | | target | insert |
|
| Sequence information |
|---|
| Length: 112 BP, A Count: 23, C Count: 19, T Count: 27, G Count: 17 |
cttatcgtta tgctatttgc aactttagan catatactaa ctcatatctc tttctcaaca 60
gcaggtgggt nnnnnnnnnn nnnnnnnnnn nnnnnaggag gatcgcgagc ca 112
|
